Visualization of Phytophthora palmivora Infection in Oil Palm Leaflets with Fluorescent Proteins and Cell Viability Markers.
Identifieur interne : 000347 ( Main/Exploration ); précédent : 000346; suivant : 000348Visualization of Phytophthora palmivora Infection in Oil Palm Leaflets with Fluorescent Proteins and Cell Viability Markers.
Auteurs : Juan C. Ochoa [Colombie] ; Mariana Herrera [Colombie] ; M Nica Navia [Colombie] ; Hernán Mauricio Romero [Colombie]Source :
- The plant pathology journal [ 1598-2254 ] ; 2019.
Abstract
Bud rot (BR) is the most devastating disease affecting oil palm (Elaeis guineensis) crops in Colombia. Its causal agent, Phytophthora palmivora, initiates the infection in immature oil palm leaflets producing necrotic lesions, followed by colonization of opportunistic necrotrophs, which increases disease damage. To improve the characterization of the disease, we transformed P. palmivora using Agrobacterium tumefaciens-mediated transformation (ATMT) to include the fluorescent proteins CFP-SKL (peroxisomal localization), eGFP and mRFP1 (cytoplasmic localization). The stability of some transformants was confirmed by Southern blot analysis and single zoospore cultures; additionally, virulence and in vitro growth were compared to the wild-type isolate to select transformants with the greatest resemblance to the WT isolate. GFP-tagged P. palmivora was useful to identify all of the infective structures that are commonly formed by hemibiotrophic oomycetes, including apoplastic colonization and haustorium formation. Finally, we detected cell death responses associated with immature oil palm tissues that showed reduced susceptibility to P. palmivora infection, indicating that these tissues could exhibit age-related resistance. The aim of this research is to improve the characterization of the initial disease stages and generate cell biology tools that may be useful for developing methodologies for early identification of oil palm materials resistant or susceptible to BR.
DOI: 10.5423/PPJ.OA.02.2018.0034
PubMed: 30828276
PubMed Central: PMC6385658
Affiliations:
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Ochoa</name><affiliation wicri:level="1"><nlm:affiliation>Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA), Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA), Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Herrera, Mariana" sort="Herrera, Mariana" uniqKey="Herrera M" first="Mariana" last="Herrera">Mariana Herrera</name><affiliation wicri:level="1"><nlm:affiliation>Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA), Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA), Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Navia, M Nica" sort="Navia, M Nica" uniqKey="Navia M" first="M Nica" last="Navia">M Nica Navia</name><affiliation wicri:level="1"><nlm:affiliation>Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA), Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA), Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Romero, Hernan Mauricio" sort="Romero, Hernan Mauricio" uniqKey="Romero H" first="Hernán Mauricio" last="Romero">Hernán Mauricio Romero</name><affiliation wicri:level="1"><nlm:affiliation>Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA), Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA), Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Departamento de Biología, Universidad Nacional de Colombia, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Departamento de Biología, Universidad Nacional de Colombia, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author></analytic><series><title level="j">The plant pathology journal</title><idno type="ISSN">1598-2254</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bud rot (BR) is the most devastating disease affecting oil palm (<i>Elaeis guineensis</i>) crops in Colombia. Its causal agent, <i>Phytophthora palmivora</i>, initiates the infection in immature oil palm leaflets producing necrotic lesions, followed by colonization of opportunistic necrotrophs, which increases disease damage. To improve the characterization of the disease, we transformed <i>P. palmivora</i> using <i>Agrobacterium tumefaciens</i>-mediated transformation (ATMT) to include the fluorescent proteins CFP-SKL (peroxisomal localization), eGFP and mRFP1 (cytoplasmic localization). The stability of some transformants was confirmed by Southern blot analysis and single zoospore cultures; additionally, virulence and in vitro growth were compared to the wild-type isolate to select transformants with the greatest resemblance to the WT isolate. GFP-tagged <i>P. palmivora</i> was useful to identify all of the infective structures that are commonly formed by hemibiotrophic oomycetes, including apoplastic colonization and haustorium formation. Finally, we detected cell death responses associated with immature oil palm tissues that showed reduced susceptibility to <i>P. palmivora</i> infection, indicating that these tissues could exhibit age-related resistance. The aim of this research is to improve the characterization of the initial disease stages and generate cell biology tools that may be useful for developing methodologies for early identification of oil palm materials resistant or susceptible to BR.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30828276</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1598-2254</ISSN><JournalIssue CitedMedium="Print"><Volume>35</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>Feb</Month></PubDate></JournalIssue><Title>The plant pathology journal</Title><ISOAbbreviation>Plant Pathol J</ISOAbbreviation></Journal><ArticleTitle>Visualization of <i>Phytophthora palmivora</i> Infection in Oil Palm Leaflets with Fluorescent Proteins and Cell Viability Markers.</ArticleTitle><Pagination><MedlinePgn>19-31</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.5423/PPJ.OA.02.2018.0034</ELocationID><Abstract><AbstractText>Bud rot (BR) is the most devastating disease affecting oil palm (<i>Elaeis guineensis</i>) crops in Colombia. Its causal agent, <i>Phytophthora palmivora</i>, initiates the infection in immature oil palm leaflets producing necrotic lesions, followed by colonization of opportunistic necrotrophs, which increases disease damage. To improve the characterization of the disease, we transformed <i>P. palmivora</i> using <i>Agrobacterium tumefaciens</i>-mediated transformation (ATMT) to include the fluorescent proteins CFP-SKL (peroxisomal localization), eGFP and mRFP1 (cytoplasmic localization). The stability of some transformants was confirmed by Southern blot analysis and single zoospore cultures; additionally, virulence and in vitro growth were compared to the wild-type isolate to select transformants with the greatest resemblance to the WT isolate. GFP-tagged <i>P. palmivora</i> was useful to identify all of the infective structures that are commonly formed by hemibiotrophic oomycetes, including apoplastic colonization and haustorium formation. Finally, we detected cell death responses associated with immature oil palm tissues that showed reduced susceptibility to <i>P. palmivora</i> infection, indicating that these tissues could exhibit age-related resistance. The aim of this research is to improve the characterization of the initial disease stages and generate cell biology tools that may be useful for developing methodologies for early identification of oil palm materials resistant or susceptible to BR.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ochoa</LastName><ForeName>Juan C</ForeName><Initials>JC</Initials><AffiliationInfo><Affiliation>Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA), Bogotá, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Herrera</LastName><ForeName>Mariana</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA), Bogotá, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Navia</LastName><ForeName>Mónica</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA), Bogotá, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Romero</LastName><ForeName>Hernán Mauricio</ForeName><Initials>HM</Initials><Identifier Source="ORCID">0000-0002-0760-2296</Identifier><AffiliationInfo><Affiliation>Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA), Bogotá, Colombia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Departamento de Biología, Universidad Nacional de Colombia, Bogotá, Colombia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>02</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>Korea (South)</Country><MedlineTA>Plant Pathol 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Ochoa</name></noRegion><name sortKey="Herrera, Mariana" sort="Herrera, Mariana" uniqKey="Herrera M" first="Mariana" last="Herrera">Mariana Herrera</name><name sortKey="Navia, M Nica" sort="Navia, M Nica" uniqKey="Navia M" first="M Nica" last="Navia">M Nica Navia</name><name sortKey="Romero, Hernan Mauricio" sort="Romero, Hernan Mauricio" uniqKey="Romero H" first="Hernán Mauricio" last="Romero">Hernán Mauricio Romero</name><name sortKey="Romero, Hernan Mauricio" sort="Romero, Hernan Mauricio" uniqKey="Romero H" first="Hernán Mauricio" last="Romero">Hernán Mauricio Romero</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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